The British driver has outperformed his teammate at each of the previous five race weekends.
The McLaren driver edged out fellow driver and title rival the Australian for the fastest time in the dying seconds of practice at the Sao Paulo Grand Prix.
Norris, who grabbed the points lead from his teammate by one point after taking the win in Mexico City two weeks ago, topped the Australian by a mere 0.023s.
Before then, Piastri had been the fastest McLaren driver during the running before sprint qualifying due at the evening session.
Max Verstappen, the other racer in the championship fight, trailing 36 points behind Norris, finished in P17 as his Red Bull team ran a different programme.
Verstappen's team-mate Yuki Tsunoda was the only driver to go off, albeit lightly, when he rotated at corner four soon after starting.
The Japanese lost control over the exit kerbs before spinning across the run-off area, and was lucky to avoid just a slight brush of the fence.
Ferrari's the seven-time champion also spun, at the conclusion of the practice, spinning around through the fast downhill corner before the last turn on to the main straight.
The seven-time champion ended the session in P19, just behind team-mate Charles Leclerc.
The McLaren drivers were far ahead of the rest of the field, with Nico Hulkenberg's Sauber third fastest, 0.619 seconds off the Australian's time.
Aston Martin's the Spanish driver was fourth fastest, with the Sauber of Gabriel Bortoleto ahead of Mercedes' Russell and Alpine's Gasly and Williams' Carlos Sainz.
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